Composite

Part:BBa_K2557014:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-EF1α-PhiC31


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 566
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 499
    Illegal BamHI site found at 1417
    Illegal BamHI site found at 1503
    Illegal BamHI site found at 1750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 566
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 566
    Illegal NgoMIV site found at 584
    Illegal NgoMIV site found at 813
    Illegal NgoMIV site found at 855
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal BsaI.rc site found at 1512
    Illegal BsaI.rc site found at 1865


Design Notes

SV40 NLS and flag was added by PCR


Source

The TetO sequence was cloned from a Commercialized plasmid. The EF1α promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of PhiC31 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References